The MIIS Eprints Archive

Measuring Plant Genetic Diversity Using Inter-Simple Sequence Repeats (ISSRS)

Iannetta, Pietro (2007) Measuring Plant Genetic Diversity Using Inter-Simple Sequence Repeats (ISSRS). [Study Group Report]



Simple sequence repeats (SSRs) have great utility as they are conserved and present in all eukaryotic genomes. Here we report the use of a simple PCR with fluorescently-labelled primers to amplify inter-SSR markers (ISSRs) for diversity assessments. The use of ISSR markers does not rely upon specific genetic sequence information, or prolonged method development and may be measured rapidly using the automated equipment. The major restriction of the ISSR method is at the analysis stage, as the markers are dominant it is not possible to distinguish heterozygotes as loci. We obtained ISSR data from ca. 60 phenotypically characterised Capsella bursa pastoris L. Medic (shepherds purse)accessions that had been isolated from a diverse mix of arable field sites throughout the UK. We developed mathematical scripts for use with the free statistical software tool R (, that processed the molecular data in a binary format to estimate genetic diversity (using the Jaccard co-efficient), and that related genotype to the plant phenotypic and environmental (site specific) traits. The methodology established has the power to predict the relationship between environmental and plant morphological characteristics.

Item Type:Study Group Report
Problem Sectors:None/Other
Study Groups:Mathematics in the Plant Sciences Study Group > 1st MPSSG [Nottingham 17/12/2007 - 20/12/2007]
Company Name:Scottish Crop Research Institute
ID Code:198
Deposited By: Michele Taroni
Deposited On:08 Jan 2009
Last Modified:29 May 2015 19:49

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